Brenno Santos Leite

3.2. Quantitative Analysis Figure 8 shows representative 2D cross-sections of the 3D reconstructions for each sample after the complete image-processing sequence. The visualization of the watershed cell separation revealed a significant difference in cell size between the BS and BL samples. It should be noted that watershed cell separation is the most critical and can lead to super segmentation. New approaches such as the Deep Watershed may improve the method’s robustness [28].