SAM-DNMT3A, a strategy for induction of genome-wide DNA methylation, identifies DNA methylation as a vulnerability in ER-positive breast cancers - Nature Communications

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By Mahnaz Hosseinpour, Ling Liu, Harrison York, C. Elizabeth Caldon, Pierre-Antoine Dugué, James Dowty, Senthil Arumugam, Melissa Southey
| Nature
Abstract DNA methylation is an epigenetic mark that plays a critical role in regulating gene expression. DNA methyltransferase (DNMT) inhibitors, inhibit global DNA methylation and have been a key tool in studies of DNA methylation. A major bottleneck is the lack of tools to induce global DNA methylation. Here, we engineered a CRISPR based approach, that we initially designed, to enable site-specific DNA methylation.
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