The third SPHIRE workshopis to be held at Rice University Houston, TX. The workshop is free, but limited to 35 participants. The target audience includes graduate students, postdocs, faculty and staff. This workshop will provide intensive hands-on-training in all practical aspects of single particle cryo-EM image processing using SPHIRE. All stages of the SPHIRE pipeline will be covered, including: Additional topical lectures will be given by Steve Ludtke, Marek Kimmel, and FEI representative.