JoVE- Journal of Visualized Experiments
Research Company/Group
JoVE creates the ultimate solutions for advancing research and science education by making and publishing videos of scientific experiments from the top laboratories around the globe.
By allowing scientists, educators and students to see the intricate details of cutting-edge experiments rather than read them in text articles, JoVE increases STEM research productivity and student learning, saving their institutions time and money.
That’s why today more than 1,000 of the world’s top universities, colleges, hospitals and biotech companies depend on JoVE to maximize productivity, efficiency and successful outcomes.
JoVE creates scientific videos for two unique resources: JoVE Video Journal and JoVE Science Education Library. Source
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| Scope | International |
|---|---|
| Language | English |
| Country | United States of America |
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Recent Articles
Search ArticlesÜberkritische Fluidchromatographie
Die überkritische Flüssigkeitschromatographie (SFC) ist eine Hybridtechnik mit einem Verhalten zwischen Flüssigkeits- und Gaschromatographie, bei der eine überkritische Flüssigkeit – oft Kohlendioxid – als mobile Phase verwendet wird. Überkritische Flüssigkeiten werden gebildet, indem eine Substanz über ihre kritische Temperatur und ihren kritischen Druck hinaus erhitzt und unter Druck gesetzt wird.
Pancreatic Tissue Dissection to Isolate Viable Single Cells
In a recently published work5, we applied the protocol described above to explore the early stages of PDAC development using a mouse model. The mouse was genetically engineered to include the cassettes Ptf1a-CreER, LSL-Kras-G12D, LSL-tdTomato7, which allow the expression of constitutively active KRAS in acinar cells after tamoxifen injection.
Genetic Modification of Primate Cerebral Organoids
The procedures described here represent a unified protocol for the generation of cerebral organoids from different primate species with a targeted electroporation approach. This allows the ectopic expression of a GOI in a model system that emulates primate (including human) (patho)physiological neocortex development. This unified protocol for the generation of primate cerebral organoids uses the same materials (e.g., media) and protocol steps for all four primate species presented.
Passaging Cells: Cell Lines, Subculturing Methods, and Applications
Cell lines are frequently used in biomedical experiments, as they allow rapid culture and expansion of cell types for experimental analysis.
Analysis of Thermal Expansion via Dilatometry
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application.
Assessment of Chemical Toxicity in Adult Drosophila Melanogaster
Abstract Human industries generate hundreds of thousands of chemicals, many of which have not been adequately studied for environmental safety or effects on human health. This deficit of chemical safety information is exacerbated by current testing methods in mammals that are expensive, labor-intensive, and time-consuming.
Gene Expression Analyses in Human Follicles
The cryopreservation of ovarian tissue is a promising approach for preserving the fertility of cancer patients. In the clinic, thawed cortical tissue is grafted back into the patient after remission, allowing the resumption of ovarian function and fertility19,20. Besides clinical use, residual ovarian fragments may also be donated for research at the end of the storage period to study the mechanisms regulating folliculogenesis.
Through a Dog's Eyes: fMRI Decoding of Naturalistic Videos from the Dog Cortex
Abstract Recent advancements using machine learning and functional magnetic resonance imaging (fMRI) to decode visual stimuli from the human and nonhuman cortex have resulted in new insights into the nature of perception. However, this approach has yet to be applied substantially to animals other than primates, raising questions about the nature of such representations across the animal kingdom.
Methods in epigenetics and chromatin biology
Just Started Methods Collections Methods in epigenetics and chromatin biology Submit Manuscript Contact Editor Submit Abstract Guest Editors Rochelle Coulson Stanford University Dr. Rochelle Coulson obtained her PhD in Integrative Genetics and Genomics from the University of California, Davis,… Yihui Zhu 10x Genomics Dr. Yihui Zhu received her PhD in Integrative Genetics and Genomics with the Designated Emphasis in Biotechnology at the… Collection Overview Cellular identity, environment,...
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
A detailed characterization of the host immune response using this particular model is shown in previous publications8,9. A few representative results of the described protocol are depicted in this section. This model aims to induce systemic infection of S. Typhimurium by intraperitoneal injection of the bacterial culture to induce sepsis. To confirm the infection, the lysates of the liver and spleen from septic mice were spread on SS agar plates, and the number of colonies was counted.