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The actin cytoskeleton is thought to play a significant role in the platelet’s ability to withstand high shear, with an inability to form the actin nodule, lamellipodia, and stress fibres all linked to thrombus instability23,26,29. However, in vivo platelet activation and thrombus growth occurs in the presence of NO and as such we used platelet spreading and in vitro flow assays to examine the role of NO within the platelet activation process.
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