3.2. Biological Experiments Assessment of cytotoxicity of compounds 1–4 against the HepG2 and MCF-7 cell lines: The Tissue Culture Unit, Department of Biochemistry, Faculty of Science, King Abdulaziz University, provided HepG2 and MCF-7 human cell lines. In complete media, Dulbecco’s Modified Eagle’s Medium (DMEM), which contains 10% fetal bovine serum and 1% antibiotic, was used to cultivate the human cell lines at 37 °C and 95% humidity in a 5% CO2 incubator for 24–48 h.
