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Abstract High-affinity O-linked β-N-acetylglucosamine (O-GlcNAc)-specific antibodies are crucial for the sensitive detection and functional study of O-GlcNAcylation. Here, we report the development of a nanomolar-affinity O-GlcNAc antibody named EPR19847 by using a degenerate O-GlcNAcylated peptide as the immunogen. The EPR19847 antibody can be used for O-GlcNAc detection, imaging, isolation, and chromatin immunoprecipitation.
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