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Using an RNA interference-based screen for HR modulators, we have previously identified USP21 as a potential positive effector of HR27 (Supplementary Fig. 1A). To validate the primary screen results and further dissect the impact of USP21 on DSB repair, we performed both overexpression and knockdown of USP21 in a DRGFP-based HR reporter cell line, which allows for doxycycline (Dox)-dependent induction of I-SceI endonuclease-mediated DSBs27.
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