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ATP5B ATP synthase F1 subunit beta CD caesarean delivery COL1A2 collagen type I alpha 2 chain COL3A1 collagen type III alpha 1 chain EIF2A eukaryotic translation initiation factor 2A group PS group ‘pregnant scarred uterus’ group PU group ‘pregnant unscarred uterus’ HPLC high-performance liquid chromatography IQR interquartile range LUS lower uterine segment ROI region of interest TEM transmission electron microscopy 1 INTRODUCTION Caesarean delivery (CD) leaves a scar in the uterus and...
Alexander Paping is a research associate at the Charité Universitätsmedizin Berlin (Germany) in the Division of ‘Experimental Obstetrics’ as well as a practicing obstetrician in the Department of Obstetrics. Here, he writes about his recent publication outlining a protocol for maximized RNA output from uterine wall samples. Often, samples for histological analysis are formalin-fixed paraffin-embedded (FFPE) and slide mounted, which introduces challenges to RNA extraction.
BioTechniquesVol. 72, No. 6 BenchmarkOpen Access Abstract Samples for histological analyses are often formalin-fixed paraffin-embedded (FFPE) and slide-mounted, which complicates RNA extraction for many downstream molecular applications. Furthermore, when the region of interest is extremely small due to isolation with laser microdissection (LMD), extracting RNA of adequate quality and quantity is difficult.
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