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We constructed a plasmid containing the PIV5 genes NP, V/P, and L that encode the NP, P, and L, respectively, with regulatory sequences (leader, trailer, and appropriate gene junctions sequences) (Fig. 1a). We inserted the EGFP gene downstream of the V/P gene as a reporter for easy tracking in live cells. To allow selection of cells containing AVLP, we inserted a selectable marker, hygromycin B resistance gene (Hyg), in the AVLP (Fig. 1a).
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