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Outcome measures Nitrite accumulation. Supernatant from cells in both models was collected after the overnight fast (i.e. 12 h of accumulation) for nitrite analysis, by way of a marker of nitric oxide (NO) production using a Sievers 280i nitric oxide analyser (NOA). Medium from a triplicate for each condition (n = 9–14 independent experiments for each) was pooled into a single tube and stored at -80°C.
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