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Bordetella pertussis is a gram-negative bacteria and the causative agent for whooping cough, a toxin mediated respiratory disease, which causes extensive damage to epithelium in the trachea and bronchial mucosa. In the absence of a human model to study vascular leukocyte recruitment to the respiratory system, and tissue injury repair, we propose an in vitro model of endothelial cells (ECs) derived from embryonic stem cells (ESCs).
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