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Abstract To establish a rat model of cerebral ischemia–reperfusion injury (CIRI) to investigate how chloride intracellular channel protein 1 (CLIC1) exacerbates neuronal damage and to assess the neuroprotective effect of its inhibition. The potential of quantitative MRI parameters (MP2RAGE T1 and R2*) as non-invasive biomarkers was also explored. Thirty-two SD rats were randomized into four groups: Sham, MCAO/R, NC + MCAO/R, and CLIC1-GS.
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