With increasing interest in faba bean as a plant-based protein crop, reliable methods to characterize protein composition are crucial for understanding structural and functional properties of faba bean-based raw materials and ingredients. A novel method combining size-exclusion high-performance liquid chromatography (SE-HPLC) with peak deconvolution was developed to separate and relatively quantify 7S and 11S globulins and other faba bean proteins at their monomer/subunit level.