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Articles by Atherly Pennybaker
07.12.22 -- Solving Bioassay Challenges For CGTs
By Minkyung Kim, Pharm.D., MBA, M.S., USP; Nikhil Rautela, M.Sc., M.Phil., USP; Shubhrata Khedkar, M.S., USP This article recaps USP's bioassay training workshop that included regulatory considerations, analytical validation, etc. related to bioassays for CGT products that are also used for potency.
Virus Production Using VERO Cells In Flasks Without Serum In Chemically Defined Cell Culture Media
By Atherly Pennybaker, Media Formulation and Product Applications Specialist, InVitria VERO cells are one of the most common continuous cell lines used in laboratory and industrial settings. VERO cells are traditionally grown in 10% fetal bovine serum (FBS) and exhibit commercially acceptable doubling times and viability.
03.29.22 -- Kite's Ultimate Supply Chain Test
Article | By Patrick Stragier, Catalent In this review, we discuss a few attributes that should be considered when planning manufacturing at scale for your cell therapy. Article | By Jonathan Carson, RoosterBio While it’s well and good to track the known critical quality attributes (CQAs), this industry needs to do a better job to diminish the impact of “unknown” outputs through stringent process control over “knowns” via the process.
Expansion Of Adherent HEK293t Cells In Flasks In Chemically Defined Cell Culture Media
By Atherly Pennybaker, Media Formulation and Product Applications Specialist, InVitria For many years, HEK293 cells have been widely accepted in the biotechnology industry due to their remarkable susceptibility for transfection and production of clinically relevant viruses or therapeutic proteins. As the growing field of gene therapy has continued to show promise in the clinic, the demand for a scalable vector production platform with HEK293t has increased as well.
02.01.22 -- CGT Validation Challenges: Proposed Solutions
Article | By Dr. Martin Schilling, Evonik Corporation The market for biological drugs has continued to grow at an impressive pace over the past years. Explore three key trends that will underpin the sustainable growth of biopharma beyond COVID-19.
OptiPEAK T Lymphocyte XPR Expansion Of T Cells Without Serum In Chemically Defined Media
By Atherly Pennybaker, Media Formulation and Product Applications Specialist, InVitria Major components used to expand human T cells include human and bovine serum, as well as proteins derived from these substances. These components not only have the potential to introduce harmful pathogens to the already compromised patient in a clinical setting, but they also have significant limitations in the ability to ensure robust and reproducible performance in cell culture systems.
Developing Students’ Understanding of Industrially Relevant Economic and Life Cycle Assessments
Training future leaders to understand life cycle assessment data is critical for effective research, business, and sociopolitical decision-making. However, the technical nature of these life cycle reports often makes them challenging for students and other nonexperts to comprehend.
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