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Abstract Dynamic contrast enhanced photoacoustic imaging (DCE PAI) can measure vascular perfusion in preclinical tumor models by monitoring the wash-in and wash-out of an exogenous contrast agent in tumor tissue. Indocyanine green (ICG) is often used for DCE PAI studies, but this agent is amphiphilic and non-specifically binds to proteins, complicating pharmacokinetics analyses of tumor vascular perfusion. A fluorescent agent, ZW800-1, may be repurposed for PAI.
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