Approximately 30 g of fresh feces were collected from sheep and thoroughly homogenized in a mortar. Saturated saline solution was added and mixed well to create a fecal suspension. The mixture was then filtered through a 0.25 mm mesh sieve for preliminary removal of large debris, followed by sequential filtration through 0.075 mm and 0.050 mm sieves to further eliminate fecal residues. The filtrate was collected, and 100 mL of 20% (w/v) sucrose solution was added.
