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Open AccessProtocol by Bayan Mashahreh , Shir Armony and Tommer Ravid * Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel * Author to whom correspondence should be addressed.
Plasmids, yeast strains, and antibodies Antibodies, yeast strains, and plasmids used in this study are listed in Supplementary Tables 2, 3, and 4, respectively. Parental plasmid for yGPS-P screen Plasmid pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR was obtained from Addgene (#24378)24 and was used as a template for PCR cloning of ADH700-yeCherry-P150-yeGFP into pTR141215 at NotI and XmaI restriction sites.
Abstract Effective proteome homeostasis is key to cellular and organismal survival, and cells therefore contain efficient quality control systems to monitor and remove potentially toxic misfolded proteins. Such general protein quality control to a large extent relies on the efficient and robust delivery of misfolded or unfolded proteins to the ubiquitin-proteasome system.
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