7H9 Middlebrook 7H9 broth 7H11 Middlebrook 7H11 agar ATc anhydrotetracycline attB bacterial attachment site attP phage attachment site BRED bacteriophage recombineering of electroporated DNA BRIP bacteriophage recombineering of electroporated DNA with in-plating selection CFU colony-forming unit CRISPR Clustered Regularly Interspaced Short Palindromic Repeats DI water deionized water gDNA genomic DNA IDT Integrated DNA Technologies LB Luria broth MAP Mycobacterium avium subsp.
