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Abstract Previously, we reported the generation of founder F0 cattle and F1 offspring harboring a targeted mutation in the prion protein gene (PRNP) using an all-in-one vector system. This study aimed to assess the long-term viability and physiological normality of F0 through comprehensive monitoring, systemic histological examinations, molecular analyses, and germline transmission.
Data Availability Statement All data and materials used in this study, including information on the filtering system, filtering rate analysis data, blocking model fitting data, particle size distribution data, Western blot data, and cell viability data, are available from the first and corresponding authors upon reasonable request. Supporting Information Filename Description bit70128-sup-0001-MilkPEP_SI.docx5 MB MilkPEP_SI. References , , , and . 2008.
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