Objectives Comparison of uterine contractility (UC) in adenomyosis patients (AP) with and without hormonal contraception (HC) compared to healthy controls with HC, measured by transvaginal ultrasound (TVUS). Methods This study is part of an ongoing multicentre prospective observational cohort study investigating UC on TVUS. 36 women with sonographic suspicion of adenomyosis without HC, 15 women with sonographic suspicion of adenomyosis with HC and 17 women with healthy uteri with HC were included.