Abstract Due to its high recurrence and pronounced metastatic potential, bladder cancer (BC) remains a significant and persistent clinical challenge. Here, we investigate the anti-tumour effects of 4-Methoxydalbergione (4MOD), a naturally occurring flavonoid obtained from Dalbergia sissoo Roxb. In T24 human and MBT2 mouse BC cell lines, 4MOD dose-dependently suppressed proliferation, migration, and invasion; colony formation was additionally suppressed in T24 cells.