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Ciara Viola

As seen in: Wiley Online Library, PLOS ONE
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Articles by Ciara Viola

Activation of GCaMP6f-Expressing Chromaffin and Satellite Glial Cells in Murine Adrenal Slices by Nanosecond Electric Pulses

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By Ciara Viola, Lisha Yang, Vasilii Mansurov
| Wiley Online Library Verified
[Ca2+]i intracellular calcium ACC adrenal chromaffin cells BBS bicarbonate-buffered saline Cd2+ cadmium CEP conventional electric pulses DMPP 1,1-dimethyl-4-piperazinium DNAse deoxyribonuclease DTT dithiothreitol E-field electric field FBS fetal bovine serum FDTD Finite Difference Time Domain GFP green fluorescent protein HBSS hanks Ca2+/Mg2+-free balanced salt solution nAChR nicotinic cholinergic receptors NEP nanosecond electric pulses PBS phosphate-buffered saline PFA paraformaldehyde ROI...
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High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca2+ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice (pdf) | Paperity

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By Ciara Viola, Thomas Gould, Nicole Procacci, Normand Leblanc
| paperity.org
PLOS ONE, Mar 2023 This is a preview of a remote PDF: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0283736&type=printable Ciara Viola, Thomas W. Gould, Nicole Procacci, Normand Leblanc, Josette Zaklit, Gale L. Craviso. , PLOS ONE, 2023, Volume 18, Issue 3, DOI: 10.1371/journal.pone.0283736
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High signal-to-noise imaging of spontaneous and 5 ns electric pulse-evoked Ca2+ signals in GCaMP6f-expressing adrenal chromaffin cells isolated from transgenic mice

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By Thomas Gould, Nicole Procacci, Normand Leblanc, Ciara Viola
| PLOS ONE
Ca2+ response kinetics. Our next goal was to test whether 5 ns electric pulses evoked Ca2+ responses in murine ACC similar to those reported for bovine ACC [25–27,42]. For this determination, wt murine ACC were loaded with Calcium Green-1 and exposed to a single 5 ns pulse using the same experimental approach and pulse exposure setup as in previous studies of bovine ACC [42,43]. Cells were first exposed to 50 μM DMPP to ensure that target cells were indeed ACC.
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