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Sialic acid (Neu5Ac, 1) is a nine-carbon α-keto acid monosaccharide that caps cellular glycoconjugates on mammalian cells. (1) Addition of Neu5Ac to the underlying glycan is catalyzed by a family of enzymes called sialyltransferases (STs), (2) which use cytidine monophosphate sialic acid (CMP-Neu5Ac, 2) as their Leloir donor, to transfer 1 to a specific hydroxyl group on a glycan acceptor (Figure S1a).
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