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Abstract N1-methyladenosine (m1A) is a recently identified mRNA methylation mark that affects gene expression and translation. In the model plant Arabidopsis thaliana, its landscape and function remain uncharacterized. Here we present base-resolution m1A methylomes across diverse Arabidopsis tissues and show that m1A is enriched in the 5′ untranslated region and preferentially localizes to highly expressed genes, negatively correlating with mRNA translation.
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