RESEARCH ARTICLE The ARRIVE Guidelines have been adopted. Dan-Yi Zeng, Zhan Chen, Mei-Zhu Hong, and Ling-Ping Jiang contributed equally to this study. Abstract Traditional Chinese medicine (TCM) is often used as an adjuvant or alternative therapy for abnormal liver biochemistry or liver fibrosis associated with chronic hepatitis B (CHB). However, the role of TCM in HBsAg seroclearance remains unclear. We aimed at exploring the role and possible mechanisms of TCM in HBsAg seroclearance.