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Articles by Daniel Heath
Gene transcriptional profiling of osmoregulatory stress response in salmonids: Development and assessment of high-throughput multispecies assays
Loading metrics Open Access Peer-reviewed Research Article Citation: Tugui N, Islam SS, Heath DD (2026) Gene transcriptional profiling of osmoregulatory stress response in salmonids: Development and assessment of high-throughput multispecies assays. PLoS One 21(10): e0360513. https://doi.org/10.1371/journal.pone.0360513 Editor: Arnar Palsson, University of Iceland, ICELAND Received: March 28, 2026; Accepted: September 28, 2026; Published: October 9, 2026 Copyright: © 2026 Tugui et al.
What Your Team Should Know About Nonprofit Web Hosting
Website Design September 29, 2026 | 5 min Read Picture this: your organization’s GivingTuesday campaign is finally live. Your supporters visit your site in droves, donations begin to roll in, and members of your marketing team are sharing the campaign link wherever they can. But all of a sudden, your donation page times out, your form won’t load, and people who were excited to give to your organization are now staring at a frozen screen or spinning wheel.
Ontario, Canada Court Of Appeal Confirms That ESA-Compliant Temporary Layoff Clauses Are Not Termination Clauses
The Ontario Court of Appeal confirmed that an ESA-compliant temporary layoff clause is not a termination clause and may remain enforceable even where a separate termination provision is invalid. The decision narrows the reach of Waksdale by focusing on the substance and legal effect of the clause, rather than its contractual heading or placement. For employers, the case underscores the value of clear temporary layoff language that expressly aligns with ESA requirements.
Ontario, Canada Court of Appeal Confirms that ESA-Compliant Temporary Layoff Clauses Are Not Termination Clauses
In a significant decision for Ontario employers, the Court of Appeal for Ontario has confirmed that a properly drafted contractual right to impose a temporary layoff in accordance with the Ontario Employment Standards Act, 2000 (ESA) is not a termination provision and is not rendered unenforceable merely because a termination clause in the employment agreement violates the ESA.
Prince Daddy & The Hyena: Cosmic Thrill Seeking Forever
Following Prince Daddy & The Hyena’s self-titled album – a dark, brooding exploration of existential dread, mortality, and the fear of impermanence – this new project takes a sharp right-turn into the light. “It wasn’t deliberately more light-hearted,” ponders Kory, “It was just a no-strings-attached approach this time around. I wanted to write a ‘singles record’,” he continues with air quotations.
Genes, genetics, and genomics in fish conservation and fisheries
Introduction Freshwater fish provide important economic, cultural, and ecological benefits. Productive and sustainable fish stocks provide jobs through commercial and recreational fisheries and provide ecological benefits through ecosystem services (De Groot et al. 2002; Quinn et al. 2018).
Using environmental DNA metabarcoding to determine endangered redside dace occurrence and associated community composition in urban streams
Get full access to this article View all available purchase options and get full access to this article. References Balasingham K.D., Walter R.P., Mandrak N.E., Heath D.D. 2018. Environmental DNA detection of rare and invasive fish species in two Great Lakes tributaries. Mol. Ecol. 27(1): 112–127. Beng K.C., Corlett R.T. 2020. Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects. Biodivers. Conserv. 29(7): 2089–2121.
Development of multi-species qPCR assays for a nanofluidic transcriptional profiling chip to assess responses of salmonids to changing environments
Get full access to this article View all available purchase options and get full access to this article. References Akbarzadeh A., Günther O.P., Houde A.L., Li S., Ming T.J., Jeffries K.M., et al. 2018. Developing specific molecular biomarkers for thermal stress in salmonids. Bmc Genomics [Electronic Resource], 19: 1–28. Allendorf F.W., Knudsen K.L., Leary R.F. 1983. Adaptive significance of differences in the tissue-specific expression of a phosphoglucomutase gene in rainbow trout. Proc. Natl. Acad. Sci.
Remembering Richard Smallwood: how his music became modern-day psalm
Some songs do more than accompany worship — they become a church’s prayer. Few modern compositions have done this as powerfully as Richard Smallwood’s “Total Praise,” a song that has crossed denominational, cultural and national boundaries to give voice to trust, lament and hope. In December 2018, while researching the Psalms as living texts of prayer, lament, and praise, I had the opportunity to interview Richard Smallwood by phone.
Stomach Content DNA (scDNA) Detection and Quantification for Predator Diet Assessment Using High‐Throughput Nanofluidic Chip Technology: Species‐Specific qPCR Assay Panel Development and Validation
Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description men14106-sup-0001-AppendixS1.docxWord 2007 document , 37.7 KB Appendix S1. References , , , et al. 2021. “Environmental DNA Reveals the Temporal and Spatial Extent of Spawning Migrations of European Shad in a Highly Fragmented River Basin.” Aquatic Conservation: Marine and Freshwater Ecosystems 31, no. 8: 2029–2040. https://doi.org/10.1002/aqc.3601. , , , , , and . 2020.
Tailoring the surface pore morphology of bioceramic scaffolds through colloidal processing for bone tissue engineering
Loading metrics Open Access Peer-reviewed Research Article Citation: Chan SSL, Heath DE, Franks GV (2025) Tailoring the surface pore morphology of bioceramic scaffolds through colloidal processing for bone tissue engineering. PLoS ONE 20(2): e0318100.
Shortest day of the year prayer
A note from the author: We refer to December 21 as the shortest day of the year even though it yields the same amount of time as the taller days. The same amount of time, just less daylight. The result is a longer night, more darkness. This day is different from the others. Daylight in Advent can remind us of Christmas’s hope, joy and peace or a spotlight on all the pain experienced in the world. Darkness in Advent often symbolizes loneliness, grief, and despair.
eDNA metabarcoding provides biodiversity estimates comparable to conventional sampling methods
Materials and methods In 2021 and 2022, preliminary fisheries surveys were conducted on stormwater ponds across the City of Hamilton by Fisheries and Oceans Canada (DFO) staff to determine fish species presence and relative abundance. Of the surveyed ponds, 14 were chosen for more intensive sampling, with priority given to ponds with more diverse fish assemblages (see Fig. 1 for pond locations). Selected ponds ranged in size from 0.013 to 1.354 ha.
Dynamic interface printing - Nature
Abstract Additive manufacturing is an expanding multidisciplinary field encompassing applications including medical devices1, aerospace components2, microfabrication strategies3,4 and artificial organs5.
Development of multi-species qPCR assays for a stress transcriptional profiling (STP) Chip to assess the resilience of salmonids to changing environments
Abstract Ecologically and socio-economically important salmonid fishes in Canada are threatened by diverse environmental stressors. However, predicting species responses to environmental change requires understanding the underlying molecular mechanisms governing environmental stress tolerance.
Genetic architecture and correlations between the gut microbiome and gut gene transcription in Chinook salmon (Oncorhynchus tshawytscha) - Heredity
Abstract Population divergence through selection can drive local adaptation in natural populations which has implications for the effective restoration of declining and extirpated populations. However, adaptation to local environmental conditions is complicated when both the host and its associated microbiomes must respond via co-evolutionary change. Nevertheless, for adaptation to occur through selection, variation in both host and microbiome traits should include additive genetic effects.
Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha)
1 INTRODUCTION Due to growth in the human population,1 an increase in production of fish for human consumption2 has been necessary to meet increasing demands,3, 4 surpassing the production levels of capture fishing practices.2, 5 To ensure sustainable food production while meeting animal welfare needs,6 advances in technologies, including producing polyploid individuals, have been employed.7 In particular, triploidization is a widespread aquaculture technique used to induce sterility to...
Hierarchically Vascularized and Implantable Tissue Constructs created through Angiogenesis from Tissue-Engineered Vascular Grafts
Abstract A major roadblock in implementing engineered tissues clinically lies in their limited vascularization. After implantation, such tissues do not integrate with the host's circulation as quickly as needed, commonly resulting in loss of viability and functionality. This study presents a solution to the vascularization problem that could enable the survival and function of large, transplantable, and vascularized engineered tissues.
Enhanced acoustic streaming effects via sharp-edged 3D microstructures
Enhanced acoustic streaming effects via sharp-edged 3D microstructures† Acoustofluidic micromanipulation is an important tool for biomedical research, where acoustic forces offer the ability to manipulate fluids, cells, and particles in a rapid, biocompatible, and contact-free manner. Of particular interest is the investigation of acoustically driven sharp edges, where high tip velocity magnitudes and strong acoustic potential gradients drive rapid motion.
Praying for kinship on Thanksgiving
The Washington Commanders (formerly the Washington Redskins) of the National Football League changed its name two years ago after acknowledging the former name was a racial slur towards native peoples. It was the combination of the name change and the book Native by Kaitlin Curtice that changed my practice of Thanksgiving. Thanksgiving is no longer only about big meals, parades, and football for me, it is also about giving thanks and lamenting for the land and the peoples of the land.
A prayer for veterans
Most veterans I know are proud of their service. I imagine that thousands who served during times of war thought they were serving in a way that did not and would not need repeating in the future. The setting of this prayer is at a Veterans Administration Hospital on Veteran’s Day.
A prayer for Labor Day: Finding a new pace
As we celebrate the many contributions of work and workers, I offer a prayer that calls us to slow down and imagine a pace of life that resists prioritizing hurry, grind, stress and production.
Researchers develop fast, inexpensive, scalable method for engineering blood vessels from natural tissue
Blood vessels keep us alive. They are the highways that transport oxygen-rich blood and nutrients to all corners of our body—feeding our tissue and organs—while simultaneously removing toxic waste products. Blood vessel disease and dysfunction can result in life-threatening situations like heart attack, stroke and aneurysm. Blood vessel failures are a major reason why cardiovascular disease is the number one killer globally. Bypass surgery is often used to replace severely diseased blood vessels.
A Father's Day confessional prayer inspired by Martin Luther King Jr.
A prayer of confession inspired by the “Letter from Birmingham Jail” written by Martin Luther King Jr. As an ordained minister in the Presbyterian Church (U.S.A.), I am aware of the significance of King’s letter as it relates to our Book of Confessions and the possibility of the letter becoming part of the Book of Confessions.
Regulation of host gene expression by gastrointestinal tract microbiota in Chinook Salmon (Oncorhynchus tshawytscha)
2.12.3 Transcriptional profile (qPCR) response to treatment The 50 selected candidate transcripts (hereafter “genes”) were tested to determine which genes showed a transcription response to either of the treatments. Two genes (cfap58, ubr4) were dropped from the analysis due to failure of PCR amplification for most of the samples, thus 48 candidate genes were included for the rest of the study.
A free hymn for Memorial Day
Did you know the complete version of the Star-Spangled Banner has four verses? Yet, we usually only sing the first verse. The other three verses further explore the complex time in America’s history that inspired Francis Scott Key to write this national treasure in 1814. I love thoughtful lyrics coupled with timeless melodies, and I love holding old treasures in new containers.
A Mother’s Day prayer inspired by womanist theology
I do not remember ever hearing about abuse in a sermon, Sunday school, Bible class or otherwise. Abuse is real, and I pray that the church can begin to be a known safe place or presence in the world for hard conversations, care, and counseling. Two works by Mitzi Smith, a new testament professor at Columbia Theological Seminary, have inspired me to write a prayer for abused women.
Towards a framework to unify the relationship between numerical abundance, biomass, and quantitative eDNA
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An ode to diverse joy
For the music score, please see “Joyful, Joyful We Adore Thee.” This hymn can be found on page #611 in Glory to God. The musical score comes from Ludwig van Beethoven’s “Symphony No. 9.” Bring to God a merry offering, all things living, bless God’s name! Praise with hearts and hands and voices, join the song of earth and heaven. Sing an anthem with the chorus, hymn God’s mercy, laud God’s grace, Play the trumpet, harp and cymbal, sound God’s love in time and space.
3D Acoustofluidics via Sub‐Wavelength Micro‐Resonators
1 Introduction Microscale manipulation of particles, cells, and cellular aggregates is an essential technique in biomedical research with applications across single-cell analysis,[1, 2] drug discovery,[3, 4] and tissue engineering.[5, 6] Micromanipulation methods such as optical tweezing, magnetophoresis, dielectrophoresis, and acoustophoresis.[7, 8] Optical tweezers, for example, are unique in that they offer a very high degree of spatial resolution, with the ability to trap and transport...
A prayer for light in Advent
Sparkling and Shimmering God, Thank you for calling us to walk in the light — light that can brighten gloomy cities, light that can make planets glow, light that can warm cold hearts. Gleaming and Glistening God, Thank you for calling us to be light — light so bright that even when we try to hide among the shrubs, burning bushes appear. Twinkling God, Thank you for calling us to share light — light that stretches beyond distances of six feet. Light that breaches depths.
When off-duty conduct crosses the line
After weeks of public protests and demonstrations related to COVID-19, many employers are asking whether they can discipline or terminate an employee for off-duty conduct. Particularly in this age of rampant social media, the internet and smartphones, an employee’s off-duty conduct has the potential to cause significant brand damage to an employer. In Vancouver, a company truck driver was recently fired after appearing to brush a cyclist at a Vancouver convoy protest.
Court reduces cost award in 'close call' wrongful dismissal case that employer won
Claims for wrongful dismissal may have become even more expensive and risky for employers thanks to a recent Ontario Superior Court of Justice decision, Goruk v Greater Barrie Chamber of Commerce.
30 years of tissue engineering, what has been achieved?
The idea of tissue engineering emerged just over 30 years ago, in 1988. The two men credited with doing the seminal work in this field are Joseph Vacanti, a surgeon, and Robert Langer, a professor at the Massachusetts Institute of Technology. However, the term “tissue engineering” was not a mainstream buzzword until almost a decade later when the infamous “Vacanti mouse” – the mouse with a human ear on its back – was revealed.
kevin.crust's 5-Star Nonprofit Story About Playground Inc
Mission: PlayGround is the Bay Area's leading playwright incubator. Our mission is to support the identification and professional development of the Bay Area's most promising emerging playwrights and, in the process, ensure the future of the American Theatre.
Restoration Success Stories
New Institute Bridges Disciplines and Institutions Across the Great Lakes A five-year grant from the US National Oceanic and Atmospheric Administration (NOAA) supports a research institute at the University of Michigan and a binational regional consortium spanning all five lakes. CIGLR research is in partnership with the NOAA Great Lakes Environmental Research Laboratory (GLERL) in Ann Arbor, where CIGLR research institute staff are housed.
Nano-scale clustering of integrin-binding ligands regulates endothelial cell adhesion, migration, and endothelialization rate: novel materials for small diameter vascular graft applications - Journal of Materials Chemistry B (RSC Publishing)
Blood contacting devices are commonly used in today’s medical landscape. However, such devices (including small diameter vascular grafts) are limited by poor blood compatibility and may fail due to thrombosis. An attractive strategy for improving the blood compatibility of such devices is to generate biomaterials that foster a confluent and functioning endothelial cell layer. Synthesizing materials that display integrin-binding peptide ligands is a common way to promote endothelialization.
Daniel Heath - The Daily Star: Obituaries
ONEONTA — Daniel Heath, 76, of Oneonta, passed away Wednesday, Oct. 28, 2015, at Bassett Medical Center in Cooperstown. He was born July 31, 1939, son of the late William Olin and Edith (Wilber) Heath. Dan served his country honorably in the United States Army from 1964 to1966. He worked as a car salesman for Cater Lincoln Mercury Sales in Yorkville for many years. He was a 26-year member of the New Hartford American Legion and VFW.
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