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Before testing the combined effect of APG and TRAIL therapy, we first evaluated the cytotoxicity of TRAIL monotherapy in two NSCLC cell lines, A549 and H1299, and a normal cell line HEK293, by means of MTT assay. Our data showed that, at the concentration of 60?ng/mL or lower, TRAIL showed no significant antitumor effect on A549 and H1299 cells, indicating that both NSCLC cell lines had low sensitivity or were resistant to TRAIL monotherapy (Fig. 1b,c).
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