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We devised a proteomics-based strategy to assess the degree of glycan processing and the degree of site occupancy of each glycosite, defined as the N-glycosylation sequon N-X-S/T (Fig. 1a). To this end, we used sequential treatment with two endoglycosidases common to glycoproteomics workflows to introduce novel mass signatures for peptides that contain the two types of glycans29,35,36.
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