Our primary objective was to determine whether ultrahigh-depth NGS sequencing with the Piseq algorithm could effectively detect meaningful variants in MM without the need for plasma cell enrichment (PiSeq-MM). To achieve this, we conducted NGS using BMA samples without performing the enrichment step. Our study cohort consisted of six MM patients who had previously undergone conventional NGS using PC. For the analysis, we used the same targeted NGS panel of 742 genes.
