APC adenomatous polyposis coli Cas9 CRISPR-associated 9 CHX cycloheximide CRISPR clustered regularly interspaced short palindromic repeats DIX dishevelled and AXIN FOP far from optimal GFP green fluorescent protein GSK3B glycogen synthase kinase 3 beta HRP horseradish peroxidase IF immunofluorescence PARsylation poly-ADP-ribosylation siRNA small interfering RNA TNKS tankyrase TOP T cell factor optimal WB western blotting WT wild-type Introduction The Wnt/β-catenin signaling pathway...