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We have recently demonstrated that fusions of different target enzymes to the coiled-coil domain TDoT induced the formation of catalytically active inclusion bodies CatIBs in E. coli (doi:10.1016/j.jbiotec.2017.04.033). Here we show that the CatIB properties can be tailored to the requirements of different reaction systems using two different coiled-coil domains as fusion tags: TDoT and 3HAMP.
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