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Analyses of non-classical monocytes When compared with H-C- controls, women with HIV had altered gene expression in NCM irrespective of whether or not they had comorbid CVD. As was found for IM, in NCM the H+C+ group had the largest set of DEGs (versus H-C-, 90 DEGs, 64 up-regulated and 26 down-regulated, Fig 2A and 2B, S4 Table).
Methods and Results: Using single-cell RNA-sequencing of aortic leukocytes from chow diet– and Western diet–fed Apoe−/− and Ldlr−/− mice, we detected 11 principal leukocyte clusters with distinct phenotypic and spatial characteristics while the cellular repertoire in healthy aortas was less diverse.
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