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Introduction Active droplets are membraneless compartments with life-like behaviors such as growth, decay, and self-organization driven by internal chemical reactions.
Abstract Bottom-up synthetic biology seeks to engineer a cell from molecular building blocks. Using DNA nanotechnology, building blocks, such as cytoskeletons, have been reverse-engineered. However, DNA nanostructures rely on chemical synthesis and thermal annealing, and therefore synthetic cells cannot produce them from their constituents such as nucleotides.
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