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Original Article Cytology and cell block immunohistochemistry of circulating tumour cells Frederick George Mayall Corresponding Author E-mail address: DrMayall@me.com Department of Cellular Pathology, Musgrove Park Hospital, Taunton, Somerset, UK Full name, postal address, e‐mail and telephone number of the corresponding author Dr Frederick George Mayall, Department of Cellular Pathology, Musgrove Park Hospital, Taunton, Somerset, UK.
Abstract Objective The study set out to assess the feasibility of using ParsortixTM circulating tumour cell (CTC) extraction and CytoFoam Disc cell‐block immunohistochemistry to diagnose metastatic carcinoma from blood samples in a National Health Service district general hospital.
We describe a method for preparing a cell block from a very sparsely cellular cytology fluid sample. The aim is to ensure that cells are not lost during processing. We developed this method to perform immunohistochemistry on circulating tumour cells (CTC) extracted from the blood of patients with metastatic carcinoma, in which there are typically less than 20 cells in a 10 mL blood sample.
We describe a method for preparing a cell block from a very sparsely cellular cytology fluid sample. The aim is to ensure that cells are not lost during processing. We developed this method to perform immunohistochemistry on circulating tumour cells (CTC) extracted from the blood of patients with metastatic carcinoma, in which there are typically less than 20 cells in a 10 mL blood sample.
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