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INTRODUCTION The development of germ cells requires genes that regulate their specification, migration and differentiation. To sustain this development, germline genes are expressed in a temporally and spatially regulated manner across these distinct stages. Among these genes, vasa (vas), encoding a DEAD-box RNA helicase, has been recognised as a highly conserved germline marker in many insects and other animals (Chen et al., 2025; Raz, 2000).
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