Abstract Macrobrachium rosenbergii is a major global aquaculture species, but its large (~3-4 Gb), highly heterozygous and repeat-rich genome has resulted in fragmented draft assemblies, limiting accurate gene annotation and functional genomics. High-quality, isoform-resolved full-length transcripts are therefore essential for gene discovery and downstream applications. Here, we present a dataset of full-length transcripts generated from seven tissues of an adult female M.