Abstract Background: Antimicrobial resistance (AMR) is an escalating global public health crisis, yet regional disparities remain poorly characterized. This study systematically assessed AMR burden in South Asia and High-income Asia-Pacific (1990–2021) to inform targeted control strategies. Methods: We analyzed data from the Global Burden of Antimicrobial Resistance Study 2021, which serves as the most advanced and current database for assessing the burden of AMR.