Chronic red blood cell (RBC) transfusions are essential for treating conditions such as sickle cell disease and β-thalassaemia. Neocyte-enriched blood may prolong the interval between transfusions. The results of this study have shown no significant difference in RBC storage lifespan between neocyte-enriched blood and filtered blood. These results highlight the need to reassess the use of neocyte-enriched blood in chronic transfusions and explore more cost-effective collection methods.