To isolate bacteria from grapevine leaves and vineyard soil and evaluate their putative modes of action against the fungus Botrytis cinerea which causes grape bunch rot. In this study, we isolated 188 bacteria from soil and leaves, collected from two vineyards in Stellenbosch. Dual culture assays were used to screen the isolates against three Botrytis cinerea strains. Nine isolates (8 Bacillus species and 1 Pantoea agglomerans) with ≥60% antifungal activity against the three B.