4. Materials and Methods 4.1. Strain and Culture Conditions D. radiodurans strains were purchased from the China General Microbiological Culture Collection Center (No. 1.3828, CGMCC, Beijing, China). The strains were cultured in TGY liquid medium (1% tryptone, 0.5% glucose, and 0.1% yeast extract) at 30 °C with shaking at 200 r/min. 4.2. Pre-Irradiation Preparation, Irradiation, and Post-Irradiation Treatment D. radiodurans strains were cultivated to the early stationary phase (OD600 = 1.5).