Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description bit70084-sup-0001-SI_information-update.docx262.6 KB Figure S1: Identification of potent endogenous promoters in B. subtilis. Figure S2: Secretion efficiency of classical and non-classical signals. Figure S3: Size Exclusion Chromatography (SEC) and Standard Curve for Molecular Weight Determination. Table S1: Primers used in this study. Table S2: Plasmid used in this study.