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Shoot tips and stem cuttings from in vitro-grown hybrid poplar (Populus tremula × alba INRA clone 717 1B4) were used as explants for plant multiplication. The explants were cut into small segments (10–15 mm long) and placed aseptically in Murashige and Skoog (MS) medium 1B (pH 5.7) supplemented with 2% sucrose and with indole acetic and indole butyric acids (0.5 mg/L) containing 0.7% (w/v) plant agar.
Abstract Poplar trees use photoperiod as a precise seasonal indicator, synchronizing plant phenology with the environment. Daylength cue determines FLOWERING LOCUS T 2 (FT2) daily expression, crucial for shoot apex development and establishment of the annual growing period. However, limited evidence exists for the molecular factors controlling FT2 transcription and the conservation with the photoperiodic control of Arabidopsis flowering.
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