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Abstract Oestradiol establishes neural sex differences in many vertebrates1,2,3 and modulates mood, behaviour and energy balance in adulthood4,5,6,7,8. In the canonical pathway, oestradiol exerts its effects through the transcription factor oestrogen receptor-α (ERα)9. Although ERα has been extensively characterized in breast cancer, the neuronal targets of ERα, and their involvement in brain sex differences, remain largely unknown.
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