Unless specifically mentioned all commercial chemicals tris(2-carboxyethyl)phosphine (TCEP), ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), sodium azide (NaN ), triethanolamine hydrochloride (TEA), tris, sodium chloride, ampicillin, isopropyl β-D-1-thiogalactopyranoside (IPTG), magnesium chloride, imidazole, 2-(N-morpholino)ethanesulfonic acid (MES), and lysozyme were obtained from Merck Sigma-Aldrich (Darmstadt, Germany), Potassium chloride was obtained from Honeywell Riedel...
