Abstract Background Digital anthropometry is increasingly accessible due to commercial availability of three-dimensional optical scanners (3DO). Methods One hundred and seventy-nine participants were assessed by four 3DO systems (FIT3D®, Size Stream®, Styku®, and Naked Labs®) in duplicate, air displacement plethysmography (ADP), and dual-energy x-ray absorptiometry (DXA). Test–retest precision was evaluated, and validity of total and regional volumes was established.