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Input–Output Relationships of Boolean Networks A cell should properly respond to extracellular stimuli to maintain homeostasis: this characteristic of a cell is defined as the input-output (IO) relationship and is determined by the dynamics of its intracellular molecular network.[16] In general, an input node of a network is defined as a node without any upstream nodes, while an output node is defined as a node without any downstream nodes.
The ultimate goal of systems biology is to control a cellular state which is determined by the dynamics of the underlying molecular regulatory network. Here, the cellular state transition dynamics is governed by both topology (structural information on interaction between molecules) and regulatory functions (operations of the types of interactions).
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