Abstract Alternaria species are among the most destructive phytopathogenic fungi affecting tomato production, with notable implications for crop yield and food safety. In this study, fourteen lactic acid bacteria (LAB) isolates from traditional Iranian fermented dairy products were screened for their probiotic and biocontrol potential. Two strains, Lactiplantibacillus pentosus ABRIIFBI50 and L. plantarum ABRIIFBI51, showed the highest antifungal activity against Alternaria solani and A.