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Rat aSMCs (Lonza Walkersville, Inc., Walkersville, MD) or mesenteric arterial SMCs were prepared, characterized and cultured in our laboratory according to previous studies52,53,54,55,56,57. Briefly, under aseptic conditions, the vessel was carefully dissected out from the rat, cleaned of extraneous tissues under the dissecting microscope, placed in a sterile HBSS and washed.
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