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Jana Kul on Muck Rack

Jana Kul

As seen in: Cell & Gene Therapy Insights
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Articles by Jana Kul

Sensitive quantification and fragment size assessment of residual host cell DNA using digital PCR

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By Miroslav Vraneš, Anida Mesihovic Karamitsos, Jana Kul
| Cell & Gene Therapy Insights Verified
What you will learn 01 How a triplex dPCR assay using short (83 bp) and long (345 bp) amplicons at the same locus combines absolute quantification of HEK293 residual DNA with median fragment size assessment in a single reaction 02 Why an extraction-free workflow maintains picogram-level sensitivity (LOQ 4 pg/reaction) and linearity across four orders of magnitude, including directly in conditioned HEK293 media 03 What an independent evaluation with viral process samples showed about skipping...
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dPCR strategies for sensitive quantification and fragment size assessment of residual host cell DNA

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By Miroslav Vraneš, Jana Kul, Anida Mesihovic Karamitsos
| Cell & Gene Therapy Insights Verified
Live30 webinars pack the latest innovations and applications into a data-rich 30-minute session. Residual host cell DNA (resDNA) is a critical quality attribute in cell and gene therapy manufacturing, with regulatory expectations spanning both highly sensitive quantification and robust assessment of DNA fragment size.
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